Online consultation
j

Fluorescent DiD Liposomes (Anionic)

The fluorescent control liposome contains the lipophilic dye DiD incorporated into the bilayer. Fluorescent plain liposomes are a type of liposome that is labeled with a fluorescent dye, allowing for easy visualization and tracking. In drug delivery research, fluorescent liposomes are utilized to investigate the distribution, targeting, and release of therapeutic agents within biological systems. Their fluorescent properties enable real-time tracking and imaging of the liposomes, providing insights into the efficacy and mechanism of drug delivery systems. Additionally, they can be used in imaging applications such as fluorescent microscopy and bioimaging. 

• Blue liquid suspensions

• Suitable for dosing with various routes

• Real-time tracking and imaging

Product Specifications
Product Name SKU Unit Size Price(¥)

Fluorescent DiD Liposomes (Anionic)

F70101FD-A-22 mL 5245.00
Product Details

DiD Labeled Fluorescent Control Liposomes for Clophosome® -A (Anionic)

The fluorescent control liposomes contain the lipophilic dye DiD incorporated in the bilayers. It has comparable physical and chemical attributes as for the Clophosome®  except no active is encapsulated inside. It is used for tracking the cellular uptake of the liposomes by common methods like confocal, FACS, etc.  

The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is an oil at room temperature and weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. 

 

Specifications

Lipid composition: phosphatidylcholine, phosphatidylglycerol and cholesterol

Dye: 0.15 mM (0.14mg/mL)

Stability: 6 months for unopened vials

Storage: 2-8 ºC

'DiD'   DiIC18(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate) (Invitrogen D-307)  

Ex/Em: 644/665 nm

CAS Number: 127274-91-3



Original References

1. van Rooijen N, van Nieuwmegen R. Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate. An enzyme-histochemical study. Cell Tissue Res. 1984;238(2):355-8. doi: 10.1007/BF00217308. PMID: 6239690.  View Article


2. van Rooijen N, van Nieuwmegen R, Kamperdijk EW. Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate. Ultrastructural aspects of elimination of marginal zone macrophages. Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(4):375-83. doi: 10.1007/BF02912114. PMID: 2867636. View Article


3. Claassen E, Kors N, van Rooijen N. Immunomodulation with liposomes: the immune response elicited by liposomes with entrapped dichloromethylene-diphosphonate and surface-associated antigen or hapten. Immunology. 1987 Apr;60(4):509-15. PMID: 2953668; PMCID: PMC1453280. View Article


4. van Rooijen N, Kors N, ter Hart H, Claassen E. In vitro and in vivo elimination of macrophage tumor cells using liposome-encapsulated dichloromethylene diphosphonate. Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;54(4):241-5. doi: 10.1007/BF02899217. PMID: 2895535. View Article


5. van Rooijen N, Kors N, Kraal G. Characterization of cell populations, reappearing in the mouse spleen after elimination by liposome encapsulated dichloromethylene diphosphonate: reappearance of marginal zone lymphocytes is independent of marginal zone macrophages. Adv Exp Med Biol. 1988;237:889-93. doi: 10.1007/978-1-4684-5535-9_133. PMID: 2978212. View Article


6. van Rooijen N, Kors N, Kraal G. Macrophage subset repopulation in the spleen: differential kinetics after liposome-mediated elimination. J Leukoc Biol. 1989 Feb;45(2):97-104. doi: 10.1002/jlb.45.2.97. PMID: 2521666. View Article


7. Su D, Van Rooijen N. The role of macrophages in the immunoadjuvant action of liposomes: effects of elimination of splenic macrophages on the immune response against intravenously injected liposome-associated albumin antigen. Immunology. 1989 Mar;66(3):466-70. PMID: 2703258; PMCID: PMC1385238.  View Article


8. Thepen T, Van Rooijen N, Kraal G. Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice. J Exp Med. 1989 Aug 1;170(2):499-509. doi: 10.1084/jem.170.2.499. PMID: 2526847; PMCID: PMC2189410. View Article

Related Products