Control for Clophosome®-A(Anionic)

Plain Control Liposomes for Clophosome®-A (Anionic). This product of empty liposomes are made to match the physical and chemical attributes of Clophosome®-A without the active clodronate encapsulated. It should be used as a negative control for macrophage depletion studies. 

Specifications 

Appearance：    White milky suspensions    

Shelf Life：    6 months guaranteed for unopened vials at 2-8 °C    

Lipid Composition：    Phosphatidylcholine and cholesterol    

Mean Particle Size：   1.5-2 µm    

Encapsulated Drug：   None    

Buffer：    Phosphate buffered saline (20 mM sodium phosphate, 0.9 % NaCl)    

pH：    7.0-7.5

Storage：    Stored at in the dark at 4°C and NEVER be frozen.    

Shipping：    Shipped on blue ice at 4°C in insulated packages   

Note: If left idle in the refrigerator, phase separation occurs (precipitate forms at the bottom of the vial, leaving a clear solution on top). Before use, shake gently to form a homogeneous solution to avoid the formation of air bubbles.  

1. van Rooijen N, van Nieuwmegen R. Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate. An enzyme-histochemical study. Cell Tissue Res. 1984;238(2):355-8. doi: 10.1007/BF00217308. PMID: 6239690.  View Article

2. van Rooijen N, van Nieuwmegen R, Kamperdijk EW. Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate. Ultrastructural aspects of elimination of marginal zone macrophages. Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(4):375-83. doi: 10.1007/BF02912114. PMID: 2867636. View Article

3. Claassen E, Kors N, van Rooijen N. Immunomodulation with liposomes: the immune response elicited by liposomes with entrapped dichloromethylene-diphosphonate and surface-associated antigen or hapten. Immunology. 1987 Apr;60(4):509-15. PMID: 2953668; PMCID: PMC1453280. View Article

4. van Rooijen N, Kors N, ter Hart H, Claassen E. In vitro and in vivo elimination of macrophage tumor cells using liposome-encapsulated dichloromethylene diphosphonate. Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;54(4):241-5. doi: 10.1007/BF02899217. PMID: 2895535. View Article

5. van Rooijen N, Kors N, Kraal G. Characterization of cell populations, reappearing in the mouse spleen after elimination by liposome encapsulated dichloromethylene diphosphonate: reappearance of marginal zone lymphocytes is independent of marginal zone macrophages. Adv Exp Med Biol. 1988;237:889-93. doi: 10.1007/978-1-4684-5535-9_133. PMID: 2978212. View Article

6. van Rooijen N, Kors N, Kraal G. Macrophage subset repopulation in the spleen: differential kinetics after liposome-mediated elimination. J Leukoc Biol. 1989 Feb;45(2):97-104. doi: 10.1002/jlb.45.2.97. PMID: 2521666. View Article

7. Su D, Van Rooijen N. The role of macrophages in the immunoadjuvant action of liposomes: effects of elimination of splenic macrophages on the immune response against intravenously injected liposome-associated albumin antigen. Immunology. 1989 Mar;66(3):466-70. PMID: 2703258; PMCID: PMC1385238.  View Article

8. Thepen T, Van Rooijen N, Kraal G. Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice. J Exp Med. 1989 Aug 1;170(2):499-509. doi: 10.1084/jem.170.2.499. PMID: 2526847; PMCID: PMC2189410. View Article